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Bushman, Wade
 Wade Bushman, MD, PhD
Associate Professor
Research Area: Human Development/ Teratogenicity. Prostate. Sonic Hedgehog. BMP-4. Inhibition of tumor growth by cyclopamine, and Shh signaling as a therapeutic target in prostate cancer.
Home Dept: Division of Urology, Department of Surgery, School of Medicine and Public Health
Affiliated Depts: Molecular and Environmental Toxicology
Address
Divison of Urology
K6/561 Clinical Sciences Center
600 Highland Avenue
Madison, WI 53706
Phone - 608-265-8705 - Email
Research
Work in my laboratory is focused on the regulation of growth during normal prostate development and the reactivation of developmental pathways in prostatic neoplasia. Working in the mouse, we are examining the role of the Sonic hedgehog (Shh) signaling pathway and Bone Morphogenetic Protein –4 (BMP-4) in the mesenchymal-epithelial signaling that regulates prostate ductal budding. We are studying the role of Hh signaling in prostate cancer and, using a xenograft tumor model, we have shown that activation of Shh signaling increases stromal Gli gene expression and – by a paracrine mechanism – significantly accelerates tumor growth. These observations suggest that the Shh pathway may be a critically important pathway in allowing tumor cells to recruit the host stromal cells to support tumor growth. Recently, our laboratory has successfully isolated and grown prostate stem cells in culture and we are pursuing studies aimed at elucidating the role of Shh and BMP signaling in stem cell maintenance and proliferation. Finally, we have developed a mouse model of chronic prostate inflammation and are studying the role of inflammatory mediators in the development of reactive hyperplasia and prostate cancer.
Publications
- Bruxvoort KJ, Charbonneau HM, Giambernardi TA, Goolsby JC, Qian CN, Zylstra CR, Robinson DR, Roy-Burman P, Shaw AK, Buckner-Berghuis BD, Sigler RE, Resau JH, Sullivan R, Bushman W, Williams BO. Inactivation of apc in the mouse prostate causes prostate carcinoma. Cancer Res. 2007 Mar 15;67(6):2490-6.
- Gipp J, Gu G, Crylen C, Kasper S, Bushman W.Hedgehog pathway activity in the LADY prostate tumor model.Mol Cancer. 2007 Mar 7;6:19.
- Zhang J, Lipinski R, Shaw A, Gipp J, Bushman W.Lack of demonstrable autocrine hedgehog signaling in human prostate cancer cell lines. J Urol. 2007 Mar;177(3):1179-85.
- Shaw A, Bushman W.Hedgehog signaling in the prostate. J Urol. 2007 Mar;177(3):832-8
- Elkahwaji JE, Zhong W, Hopkins WJ, Bushman W. Chronic bacterial infection and inflammation incite reactive hyperplasia in a mouse model of chronic prostatitis. Prostate. 2007 Jan 1;67(1):14-21.
- Shaw A, Papadopoulos J, Johnson C, Bushman W. Isolation and characterization of an immortalized mouse urogenital sinus mesenchyme cell line. Prostate. 2006 Sep 15;66(13):1347-58.
- Doles J, Cook C, Shi X, Valosky J, Lipinski R, Bushman W. Functional compensation in Hedgehog signaling during mouse prostate development. Dev Biol. 2006 Jul 1;295(1):13-25. Epub 2006 May 16.
- Lipinski RJ, Gipp JJ, Zhang J, Doles JD, Bushman W. Related Unique and complimentary activities of the Gli transcription factors in Hedgehog signaling. Exp Cell Res. 2006 Jul 1;312(11):1925-38. Epub 2006 Mar 29.
- Doles JD, Vezina CM, Lipinski RJ, Peterson RE, Bushman W. Growth, morphogenesis, and differentiation during mouse prostate development in situ, in renal grafts, and in vitro. Prostate. 2005 Dec 1;65(4):390-9.
Check PubMed for other publications by Wade Bushman
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