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Lee, Youngsook
 Youngsook Lee, PhD
Associate Professor
Research Area: Cardiac Development. Molecular pathways controlling cardiovascular development and maintenance of normal cardiac phenotype; transcription factors and signal transduction; isolation and characterization of stem cell-derived cardiomyocytes.
Home Dept: Anatomy, School of Medicine and Public Health
Affiliated Depts: Molecular and Environmental Toxicology
Address
Department of Anatomy, School of Medicine and Public Health
1300 University Avenue
Madison, WI 53706
Phone: 608/265-6352 - Email
Research
Transcriptional control of normal cardiovascular development and function
Malformation of the cardiovascular system is the most common form of human birth defect and cardiovascular disease is the leading cause of mortality. However, the molecular mechanisms that cause these heart diseases remain poorly understood.
The long-term goal of my laboratory is to advance our understanding of the transcriptional controls that guide cardiovascular differentiation / development and maintenance of normal cardiac function.
The focus of our current research is to identify novel cellular and molecular mechanisms that lead to normal cardiac morphogenesis and function. For this, we are investigating molecular mechanisms that regulate cardiac-specific gene expression in vitro and role of transcription factors in cardiovascular development in vivo by generating transgenic mice. For example, we are studying molecular functions of the cardiac-restricted homeobox protein Nkx2.5, zinc finger protein GATA4, muscle-specific factor MEF2, and the nuclear factor Jumonji in regulating target genes and guiding normal cardiovascular development.
Embryonic stem cells are pluripotent cells. One goal of stem cell research is the development of specialized cells such as heart muscle cells. The directed differentiation of embryonic stem cells is then vital to the ultimate use of such cells in the development of new therapies. We are currently developing methods to isolate and enrich embryonic stem cell-derived cardiomyocytes.
- Kim, T.-G., Kraus, J.C., Chen, J., and Lee, Y. JUMONJI, a critical
factor for cardiac development, functions as a transcriptional
repressor. J of Biological Chemistry, 278:42247-42255, 2003. - Ganga, M., Espinoza, H.M., Cox, C.J., Morton, L., Hjalt, T.A., Lee, Y., and Amendt, B.A. PITX2 isoform-specific regulation of atrial natriuretic factor expression. J of Biological Chemistry, 278:22437-22445, 2003.
- He, J.-Q, Ma, Y., Lee, Y., Thomson, J.A., and Kamp, T.J. Human embryonic stem cells develop into multiple types of cardiac myocytes. Circulation Research. 93:32-39, 2003. (an editorial article on this paper)
- Kim, T.-G., Chen, J., Sadoshima, J., and Lee, Y. JUMONJI represses atrial natriuretic factor gene expression by inhibiting transcriptional activities of cardiac transcription factor. Molecular Cellular Biology, 24:10151-10160, 2004.
- Jung, J., Mysliwiec, M.R., and Lee, Y. The roles of JUMONJI in mouse embryonic development. Developmental Dynamics, 232:21-32, 2005.
- Kim, T.-G., Jung, J., Mysliwiec, M.R., and Kang, S., and Lee, Y. JUMONJI represses a-cardiac myosin heavy chain expression via inhibiting MEF2
activity. Biochem. Biophys. Res. Commun. 329:544-553, 2005. - Jung, J., Kim, T.-G., Lyons, G.E., Kim, H.R.C and Lee, Y. Jumonji regulates cardiomyocyte proliferation via interaction with
retinoblastoma protein. J. Biological Chemistry, 280:30916-30923, 2005. - Mysliwiec, M.R., Chen, J., Powers, P.A., Bartley, C.R., Schneider, M.D., and Lee, Y. Generation of a Conditional Null Allele of Jumonji. Genesis, 44(9):407-411, 2006.
- Mysliwiec, M.R., Kim, T.-G., and Lee, Y. Characterization of Zinc Finger Protein 496 that interacts with Jumonji/jarid2. FEBS Letters, 581(14):2633-2640, 0007.
- Wozniak R.J., Boyer, M.E., Grass, J.A., Lee, Y., and Bresnick, E.H. Context-dependent GATA factor function: Combinatorial requirements for transcriptional control in hematopoietic and endothelial cells. J of Biological Chemistry, 282(19):14665-74, 2007.
- Kim, K.-H., Antkiewicz, D.S., Yan, L., Eliceiri, K.W., Heideman, W., Peterson, R.E., and Lee, Y. Lrrc10 is required for early heart development and function in zebrafish. Developmental Biology,
308:494-506, 2007. - Kim, K.-H., Kim T.-G., Micales, B., Lyons, G.E., and Lee, Y. Dynamic expression patterns of Leucine Rich Repeat containing protein 10 in the heart. Developmental Dynamics, 236:2225-2234, 2007.
- Lin, B.C., Sullivan R., Lee, Y., Moran, S., Glover, E., and Bradfield, C.A. Deletion of aryl hydrocarbon receptor associated protein 9 leads to cardiac malformation and embryonic lethality. J. Biol. Chem.
282:35924-32. 2007.
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