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Home » ... » - Faculty » Faculty Pages » Keely, Patricia J. |
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Keely, Patricia J.
 Patricia J. Keely, PhD
Associate Professor
Research Area- Cancer Biology, Cell Structure, Extracellular matrices, Cell Migration and Cell Communication
Home Dept -
Affiliated Depts - Molecular and Environmental Toxicology; Molecular Biology; Co-PI of Laboratory for Optical and Computational Instrumentation (LOCI)
Address
School of Medicine and Public Health
3630 Medical Sciences Center
1300 University Avenue
Madison, WI 53706
Phone: 608/ 265-2398 - Email
Research - Appropriate cellular interactions with the extracellular matrix (ECM) help to establish normal cellular architecture and differentiation. During oncogenic transformation, these normal interactions with the ECM are profoundly altered, resulting in cells that lose their polarization and differentiation, lose anchorage dependent growth control, and acquire a migratory, invasive phenotype. Our lab is interested in understanding, at a molecular level, how cellular interactions with the ECM determine differentiation and epithelial polarization, and how these interactions are altered during carcinogenesis to result in invasive, metastatic carcinoma. Cells interact with the ECM through a variety of cell surface receptors, the best understood of which are members of the integrin family. Much remains to be determined regarding the specific molecular players and signaling pathways downstream of integrins, and how these pathways are involved in the progression of various diseases. Therefore, part of the focus of the lab is to investigate signaling events through the integrin family of receptors. A second aspect of this work is to investigate how small GTPases of the Ras superfamily, some of which are known or suspected oncogenes, affect the response of cells to the ECM. Specifically, we has focused on R-Ras and Rho, which we find alter the way breast epithelial cells respond to the ECM, promoting cellular migration and invasion. We are particularly interested in studying signaling events using state of the art imaging approaches to understand how small GTPases function in a spatial and temporal manner during cell migration.
Publications- Keely PJ, Conklin MW, Gehler S, Ponik SM, Provenzano PP. Investigating integrin regulation and signaling events in three-dimensional systems. Methods Enzymol. 2007;426:27-45.
- Skala MC, Riching KM, Bird DK, Gendron-Fitzpatrick A, Eickhoff J, Eliceiri KW, Keely PJ, Ramanujam N. In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia. J Biomed Opt. 2007;12:024014.
- Provenzano PP, Eliceiri KW, Campbell JM, Inman DR, White JG, Keely PJ. Collagen reorganization at the tumor-stromal interface facilitates local invasion. BMC Med. 2006 Dec 26;4(1):38.
- Modzelewska K, Newman LP, Desai R, Keely PJ. Ack1 mediates Cdc42-dependent cell migration and signaling to p130Cas. J Biol Chem. 2006; 281:37527-35.
- Pal S, Wu J, Murray JK, Gellman SH, Wozniak MA, Keely PJ, Boyer ME, Gomez TM, Hasso SM, Fallon JF, and Bresnick EH. (2006). An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. J Cell Biol. 174:1047-58.
- Ada-Nguema AS, Xenias H, Sheetz MP, and Keely PJ. (2006). The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLCepsilon. J Cell Sci. 119(Pt 7):1307-19.
- Wozniak MA and Keely PJ. (2005). Use of three-dimensional collagen gels to study mechanotransduction in T47D breast epithelial cells. Biol Proced Online. 7:144-61.
- Bird DK, Yan L, Vrotsos KM, Eliceiri KW, Vaughan EM, Keely PJ, White JG, and Ramanujam N. (2005). Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH. Cancer Res. 65:8766-73.
- Wozniak MA, Kwong L, Chodniewicz D, Klemke R, and Keely PJ. (2005). R-Ras controls membrane protrusion and cell migration through the spatial regulation of Rac and Rho. Mol Biol Cell. 16:84-96.
- Wozniak MA, Modzelewska K, Kwong L, and Keely PJ. (2004). Focal adhesion regulation of cell behavior. Biochemica Biophys Acta. 1692:103-119.
Check PubMed for other publications by Patricia J. Keely
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