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- Current Students
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Student Publications
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Bae, JuYun
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Boehm, Bayli
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Boley, Patricia
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Bolterstein, Elyse
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Brody, Matthew
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Bultman, JoAnna
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Calkins, Marcus
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Desotelle, Josh
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Dever, Joe
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Hutchinson, John
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Irving, Roy
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Johnson, Brian
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Johnson, Delinda
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Jung, Brittney
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Mehta, Vatsal
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Novick, Rachel
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Pham, Ly
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Rhoads, Keelia
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Rufer, Echoleah
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Sand, Jordan
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Schmit, Travis
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Shan, Weihua
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Shetty, Ameesha
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Tarapore, Rohinton
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Wiecinski, Paige
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Yang, Sarah
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Yu, Min
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Malone, Thomas
Thomas Malone
PhD Candidate - Started 2001
Native of Oklahoma
Lab of Brian Fox, PhD
Contact Information
Email: Thomas Malone
Undergraduate Work
Research 2005
After analyzing enzymatic TNT transformation products from three out of the four major evolutionary branches of the phylogenetic tree, patterns in functionality can begin to be inferred. Flavoprotein xenobiotic reductases from branch one may be characteristic of XenA, which catalyses the reduction of the nitro group only, producing the hydroxylamine dinitrotoluene. Those of branches three and four may have similar reductive specificity to XenB and OPR1 respectively.
These enzymes reduce TNT via both the aromatic ring and the nitro group, forming both the Meisenheimer complex and the hydroxylamine dinitrotoluene and thus the dimer product.
The genes encoding for the xenobiotic reductases that were discovered in the Envirogen soil and bioreactor samples are in the process of being cloned via the creation and screening of DNA libraries from the bacterial isolates’ genomic DNA. These genes will be sequenced and inserted into the xenobiotic reductase phylogenetic tree to assist in the further characterization of the specific functionality of each evolutionary branch.
Four more Arabidopsis thaliana genes encoding OPR isoforms are in the progress of being cloned, including OPR2, OPR3, 12-OP-10, 11-R, and a putative OPR. The proteins expressed by these genes will be purified and characterized for their TNT reduction specificities and crystallized.
Our group also plans on PCR cloning several OYE homologs from Saccharomyces cerevisiae cDNA in order to characterize the specific functionality of that particular evolutionary branch of the xenobiotic reductase phylogenetic tree
Internship
Fall 2005 internship in Chicago with Evanston Northwestern Healthcare Research Institute as part of the Biotechniology grant requirements.
Funding
Publications
Posters
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