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Rubert, Kennedy (Ned)

Ned Rubert, PhD
Graduated Spring 2008
Native of Virginia
Lab of Joel Pedersen, PhD

Contact Information
Email: Ned Rubert

Undergraduate Work
University of Virginia
Bachelor of Science, Environmental Chemistry (2002)

Memberships
American Chemical Society
Hoofer Outing Club
Alpha Chi Sigma Professional Chemistry Fraternity

Interests/Hobbies
Whitewater kayaking, skiing, hiking/camping, chemistry tutoring

Research as of August 2006
My current research project, titled "Tetracycline resistance genes in aquaculture environments: genotypic diversity and potential resistance resistance reservoirs", is being conducted in collaboration with McMahon group in Civil & Environmental Engineering with support from Sea Grant. Research is focused on the relationship between the fate and transport of oxytetracycline (OTC) and the spread of antibiotic resistance among microorganisms at aquaculture facilities in northern Wisconsin. I focus on tracking the fate of OTC in the environment while a M.S. student in the McMahon (Civil & Environmental Engineering) group concurrently tracks resistance genes in environmental bacteria using a PCR-based approach. The liquid chromatography-mass spectrometry (LC/MS) techniques employed to quantitate OTC in the aquaculture field samples are additionally being used to characterize the products formed by reactions of OTC both in vitro (with Mn oxides) and in situ (sediment microcosms). The goals of this research are to determine whether a relationship exists between antibiotic use and apparent bacterial resistance to antibiotics in aquaculture facilities, to study the reactions of OTC on Mn oxide minerals, and to identify transformation products of OTC in sediments.

Publications
  • Rubert, K.F.; Pedersen, J.A. Oxidative transformation of tetracycline antibiotics by a hydrous manganese oxide. Environ. Sci. Technol. 2006, 40, 7216-7221.

  • Liang, C.; Rubert, K.F. Effect of plant material on microbial transformation of amino sugars in soils. Biol. Fert. Soils. 2006, DOI 10.1007/s00374-006-0142-1.

  • Rubert, K.F.; Hedman, C.J.; Pedersen, J.A. Influence of MnO2 on the transformation of oxy- and chlortetracycline in pond water. ACS Symp. Ser., Agrochemicals Division [in review].
Posters/Presentations
  • Rubert, K.F.; Hedman, C.J.; Pedersen, J.A. Abiotic transformation of tetracycline antibiotics in a natural surface water and in the presence of MnO2. Am. Chem. Soc. Natl. Mtg., Chicago, IL. Mar. 25-29, 2007. AGRO 35.
  • Rubert, K.F.; Hedman, C.J.; Pedersen, J.A. MnO2-mediated transformation of oxytetracycline and chlortetracycline: Characterization of transformation products. Am. Chem. Soc. Natl. Mtg., Chicago, IL. Mar. 25-29, 2007; ENVR 172.
  • Rubert, K.F.; Pedersen, J.A. Thermal kinetics of oxytetracycline transformation with a hydrous manganese oxide. Am. Chem. Soc. Natl. Mtg., Washington, DC, Aug. 28-Sept. 1, 2005; ENVR 149.
  • Rubert, K.F.; Gao, J.; Pedersen, J.A. Mineral-mediated transformation of oxytetracycline. Annual Midwest Chapter Soc. Environ. Toxicol. Chem. Mtg, Madison, WI, April 5-6, 2005; p. 23.

  • Rubert, K.F.; Lund, K.; Meyer, M.T.; Pedersen, J.A. Accelerated solvent extraction of multiple antibiotics from soils. Soil Sci. Soc. Am. Natl. Mtg., Denver, CO, November 2-6, 2003.


                  • Photo Credit: Chris Frazee, Media Solutions


Date Last Updated: 08/27/2008 webteam@med.wisc.edu