Undergraduate education

College of St. Benedict, BA, 1995

Years in MSTP

1995-2003

PhD

Biochemistry, 2003

Mentor

M. Thomas Record Jr., PhD

Thesis title

Calorimetric Studies of DNA Helix Formation and Integration Host Factor-DNA Interactions: Contribution of Coupled Processes to Observed Thermodynamics

Fellowship

Molecular medicine, EMBL Heidelberg, Germany

Current position

Freelance medical writer, Phase I Clinical Trials, Ulm, Germany

PhD publications

Tsodikov OV, Holbrook JA, Shkel IA, Record MT Jr. Analytic binding isotherms describing competitive interactions of a protein ligand with specific and nonspecific sites on the same DNA oligomer. Biophys J. 2001 Oct;81(4):1960-9. PMID: 11566770; PMCID: PMC1301671.

Holbrook JA, Tsodikov OV, Saecker RM, Record MT Jr. Specific and non-specific interactions of integration host factor with DNA: thermodynamic evidence for disruption of multiple IHF surface salt-bridges coupled to DNA binding. J Mol Biol. 2001 Jul 6;310(2):379-401. PMID: 11428896.

Holbrook JA, Capp MW, Saecker RM, Record MT Jr. Enthalpy and heat capacity changes for formation of an oligomeric DNA duplex: interpretation in terms of coupled processes of formation and association of single-stranded helices. Biochemistry. 1999 Jun 29;38(26):8409-22. PMID: 10387087.